Post-infectious time-series DNA methylation analysis in gastric epithelial cells

Time-series comprehensive DNA methylation analysis after EBV infection in the immortalised normal gastric epithelial cell line, GES1, and low methylation gastric cancer cell line, MKN7. Infinium HumanMethylation450 BeadChip was used to obtain DNA methylation profiles across 485,577 CpG sites. Samples included 9 GES1 with and without EBV infection, 5 MKN7 with and without EBV infection, EBV+ gastric cancer cell and xenograft, SNU719 and KT, 2 normal gastric mucosae, 2 low-methylation gastric cancer cases, and 2 high-methylation gastric cancer cases. Bisulphite converted DNA from the 20 samples were hybridised to the Infinium HumanMethylation450 BeadChip.

本数据集针对永生化正常胃上皮细胞系GES1与低甲基化胃癌细胞系MKN7，开展EB病毒（Epstein-Barr virus，EBV）感染后的时序性全维度DNA甲基化分析。本研究采用Infinium HumanMethylation450 BeadChip芯片，对覆盖485577个CpG（CpG site）位点的DNA甲基化谱进行检测。所纳入的样本包括：9组GES1细胞（分别设置EBV感染与未感染实验组）、5组MKN7细胞（分别设置EBV感染与未感染实验组）、EBV阳性胃癌细胞系及异种移植瘤SNU719与KT、2例正常胃黏膜组织、2例低甲基化胃癌病例以及2例高甲基化胃癌病例。本研究对上述20份样本的亚硫酸氢盐转化DNA进行Infinium HumanMethylation450 BeadChip芯片杂交实验。