Fetal-derived activated CD83+ γδ T cells expand in preterm infants with sepsis.

Here, we investigated γδ T cells in a longitudinal cohort of preterm neonates during the first year of life. Our data reveal dynamic postnatal maturation patterns of γδ T cell subsets, which are largely age-dependent. We report on the expansion of fetal-derived γδ T cells in preterm neonates with sepsis. Single cell transcriptome analyses identified HLA-DRhigh and CD83+ γδ T cells in neonatal sepsis. Combined single-cell transcriptome (scRNA-seq) and single-cell γδTCR-seq (scTCR-seq) analysis was conducted from peripheral blood.

本研究针对出生后第一年的早产新生儿纵向队列，对γδ T细胞（γδ T cell）展开了系统性探究。研究数据揭示了γδ T细胞亚群呈现出动态的出生后成熟模式，该模式在很大程度上依赖于年龄。本研究还报道了合并脓毒症的早产新生儿体内，胎儿源性γδ T细胞发生扩增的现象。单细胞转录组分析在新生儿脓毒症样本中，鉴定出了HLA-DR高表达（HLA-DRhigh）与CD83阳性（CD83+）的γδ T细胞亚群。研究同时对外周血样本开展了单细胞转录组测序（scRNA-seq）与单细胞γδ T细胞受体测序（scTCR-seq）的联合分析。