Changes in cell cycle pattern in bone marrow and spleen erythroid cells after paraquat treatment.

Mice were treated with repeated doses (10 mg/kg) of paraquat on alternate days. Bone marrow/spleen cells were harvested after 14 days of exposure and stained with anti-mouse Ter 119-FITC and propidium iodide followed by flow cytometric analysis. Ter119+ve cells were gated and cell cycle analysis was done on gated cells by utilizing Modfit-LT software. Each value represents mean ± SEM of data from 5–6 mice.
*p<0.05 Standard t test, comparisons of cells in different phases of cell cycle in control and paraquat treated mice.

小鼠隔日接受百草枯（paraquat）的重复给药，给药剂量为10 mg/kg。暴露14天后收获骨髓与脾脏细胞，使用抗小鼠Ter119-FITC和碘化丙啶（propidium iodide）进行染色，随后进行流式细胞术分析。对Ter119阳性细胞设门，并利用Modfit-LT软件对该群设门细胞开展细胞周期分析。所有数值均为5~6只小鼠实验数据的均值±标准误（standard error of the mean, SEM）。
*p<0.05：采用标准t检验比较对照组与百草枯处理组小鼠细胞周期各时相的细胞占比，差异具有统计学显著性。