RNA-Seq based gene expression profiling of mouse Meis1-Meis2 double KO or wild-type atria and ventricles (Adult).

The goal of this study was to compare the gene expression profile, using RNA-seq technology, of mouse Meis1-Meis2 double KO or wild-type fetal or adult hearts. Overall design: RNA-seq was performed in four different conditions, atria and ventricles from fetal hearts at E15.5 and from adult hearts to compare Meis1 and Meis2 double deletion (constitutive or inducible) with control littermates. In all cases, 4 replicates were used for each condition.

本研究旨在采用RNA测序（RNA-seq）技术，对比小鼠Meis1与Meis2双基因敲除型及野生型胎鼠、成年小鼠心脏的基因表达谱。实验设计概述：本研究设置四种实验条件，分别采集胚胎期15.5天（E15.5）胎鼠心脏的心房、心室组织与成年小鼠心脏组织，以比较Meis1与Meis2双基因缺失（分为组成型敲除与诱导型敲除两类）与其同窝野生型对照的基因表达差异。所有实验条件均设置4次生物学重复。