Foxd ChIP-seq in Ciona embryos. Ciona intestinalis

Foxd binding regions were examined by chromatin immunoprecipitation followed by deep-sequencing using 32-cell embryos of Ciona intestinalis (type A). In this experiment, a Foxd-Gfp fusion gene was expressed under the control of the upstream regulatory region of Foxd, and an anti-GFP antibody was used for immunoprecipitation.

本研究以A型玻璃海鞘（Ciona intestinalis）的32细胞期胚胎为实验材料，通过先进行染色质免疫沉淀（chromatin immunoprecipitation）再辅以深度测序（deep-sequencing）的实验策略，对Foxd结合区域进行了检测。本次实验中，研究人员将Foxd-Gfp融合基因（Foxd-Gfp fusion gene）置于Foxd基因的上游调控区域（upstream regulatory region）的调控下实现表达，并使用抗GFP抗体（anti-GFP antibody）完成免疫沉淀操作。