Cephalotaxus hainanensis leave Transcriptome with and without MeJA treatment

In previous work, cephalotaxine, harringtonine, homoharringtonine were shown to be accumulated differentially after various stimuli. Especially, after MeJA treatment, the concentration of 3 cephalotaxus alkaloids all showed decreasing. We speculated that the genes expressed lower after MeJA treatment might encode some enzymes responsible for the biosynthesis of cephalotaxus alkaloids. Therefore, choosing the sample treated with MeJA and the control sample for comparative transcriptome and iTRAQ analysis will greatly facilitate dissection of the genes involved in the biosynthesis of cephalotaxus alkaloids and even the acyl portions of cephalotaxus ester alkaloids.

既往研究显示，三尖杉碱（cephalotaxine）、哈林通碱（harringtonine）与高三尖杉酯碱（homoharringtonine）在不同外界刺激下的积累水平存在显著差异。尤为值得注意的是，经茉莉酸甲酯（MeJA）处理后，上述3种三尖杉属生物碱的含量均呈下降趋势。我们推测，经茉莉酸甲酯处理后表达量下调的基因，或可编码参与三尖杉属生物碱生物合成的相关酶类。据此，选取茉莉酸甲酯处理组样本与对照组样本开展比较转录组与同位素相对与绝对定量（iTRAQ）分析，将极大助力阐明参与三尖杉属生物碱，乃至三尖杉酯类生物碱酰基部分生物合成的相关基因。