RIP1 inhibition protects TMJ from stress-induced TMJ OA

A compressive mechanical force-induced-TMJ OA rat model was used in the study. Lenti-virus targeting RIP1 was constructed in vitro and injected into the TMJ. Hematoxylin and eosin staining, TUNEL staining and micro-computed tomography were used to assess histological and morphological changes in the mandibular condyle. Immunohistochemistry, Western Blot and real-time PCR were performed to determine the changes of inflammatory, apoptotic and necroptotic markers.

本研究采用压缩机械力诱导的颞下颌关节骨关节炎（TMJ OA）大鼠模型。体外构建靶向受体相互作用蛋白1（RIP1）的慢病毒（Lenti-virus），并将其注射至颞下颌关节内。采用苏木精-伊红染色（Hematoxylin and eosin staining）、TUNEL染色（TUNEL staining）及微型计算机断层扫描（micro-computed tomography）评估下颌髁突的组织学与形态学变化。通过免疫组织化学（Immunohistochemistry）、蛋白质印迹法（Western Blot）及实时荧光定量聚合酶链式反应（real-time PCR）检测炎症、凋亡及坏死性凋亡标志物的表达变化。