Serum antibodies and viral detection in the H5N1-infected (group 3) and post acute stage contact (group 4) ducks.

Ten ducks (group 3) inoculated with H5N1 HPAI virus were mixed with nine naive contact ducks (group 4) at 10 day post inoculation (dpi). Sera were collected at 4 days pre-inoculation for both groups and at 8, 15, 22, 29, and 34 dpi for both groups, which corresponded to 12, 19 and 24 days post contact (dpc) for group 4. Sera were tested using blocking ELISA (values are % inhibition; cut-off = 60%), HI with chicken RBCs (HI-C) and horse RBCs (HI-H), and virus neutralization (VN) tests. nd: not done.
a Summary of virus isolation (VI): a+symbol indicates that virus was isolated at least once from an oral or cloacal swab from the duck during the duration of the trial.
b Summary of Ct values for real-time reverse transcription polymerase chain reaction (rRT-PCR) results: the value is the lowest detected for the duck from either oral or cloacal swabs during the duration of the trial.

10只接种H5N1高致病性禽流感病毒（H5N1 HPAI）的第3组鸭，于接种后天数（days post inoculation, dpi）10天时，与9只未接触过病毒的易感接触鸭（第4组）混群饲养。两组均于接种前4天采集血清；同时分别于接种后8、15、22、29及34 dpi时采集血清，其中第4组的对应接触后天数（days post contact, dpc）为12、19和24天。血清样本通过以下方法检测：阻断酶联免疫吸附试验（blocking ELISA）（结果以抑制率百分比计，临界值为60%）、鸡红细胞血凝抑制试验（HI with chicken RBCs, HI-C）、马红细胞血凝抑制试验（HI with horse RBCs, HI-H）以及病毒中和试验（virus neutralization, VN）。nd：未完成检测。
a 病毒分离（virus isolation, VI）结果汇总：带有"+"符号表示在试验周期内，至少从该鸭的口腔或泄殖腔拭子中分离到一次病毒。
b 实时反转录聚合酶链式反应（real-time reverse transcription polymerase chain reaction, rRT-PCR）的Ct值结果汇总：该数值为试验周期内，从该鸭口腔或泄殖腔拭子中检测到的最低Ct值。