Genome-wide analysis of c-Myb-responsive gene expression in prostate cancer cells

Analysis of c-Myb-regulation of gene expression. The hypothesis tested in the present study was that c-Myb influences the expression of specific sets of genes that are involved in cell cycle, DNA replication, recombination and repair. Results provide important information on c-Myb-responsive genes that may be crucial to the cell survival and the progression of prostate cancer. Total RNA obtained from c-Myb siRNA-transfected prostate cancer cells compared to negative control siRNA-transfected prostate cancer cells 48 h after siRNa transfection.

本研究针对c-Myb对基因表达的调控作用开展分析。本研究验证的科学假说为：c-Myb可影响参与细胞周期、DNA复制、重组与修复的特定基因集合的表达水平。本研究结果可为c-Myb应答基因提供重要参考信息，此类基因可能对细胞存活及前列腺癌的进展至关重要。实验于小干扰RNA（siRNA）转染48小时后，提取经c-Myb靶向小干扰RNA转染的前列腺癌细胞的总RNA，并与转染阴性对照小干扰RNA的前列腺癌细胞进行对比分析。