Multiple functions of Sir2-HerA system cooperate to defense viral infection, and Dongmei Tang et al.

Figure 3. ATP inhibited the helicase, nuclease, and NADase activities of Nezha. Figure 5H. Nuclease activity of purified Nezha’s Sir2 alone against single-strand DNA. Figure S6. Figure S6. The NADase and nuclease activities of Nezha. Figure S7A. Immunoblotting analysis of the expression of Nezha system during phage infection. Figure 2F. Thin-layer chromatography analysis of Nezha ATPase against ATP or dATP. Figure 2B. Nezha showed no activity on non-adenine nucleotides. Thin-layer chromatography analysis of Nezha against various non-adenine nucleotides are shown.

图3：三磷酸腺苷（ATP）可抑制哪吒蛋白（Nezha）的解旋酶（helicase）、核酸酶（nuclease）与烟酰胺腺嘌呤二核苷酸酶（NADase）活性。
图5H：单独纯化的哪吒蛋白Sir2针对单链DNA的核酸酶活性分析。
图S6：哪吒蛋白的烟酰胺腺嘌呤二核苷酸酶与核酸酶活性。
图S7A：噬菌体感染过程中哪吒蛋白系统表达情况的免疫印迹（immunoblotting）分析。
图2F：哪吒蛋白ATP酶（ATPase）针对三磷酸腺苷（ATP）或脱氧三磷酸腺苷（dATP）的薄层色谱（thin-layer chromatography）分析。
图2B：哪吒蛋白对非腺嘌呤核苷酸无活性，针对各类非腺嘌呤核苷酸的哪吒蛋白活性薄层色谱分析结果如下所示。