RNAseq of human Ewing, MSC, fibroblast cell lines - A side population of Ewing cells exhibits stem cell properties

Ewing's Sarcoma (ES) belongs to the group of bone cancers defined by the existence of a certain EWS-ETS fusion gene. In this study we use the model cell line CADO-ES1 (EWSR1-ERG fusion gene) to characterize the tumor biology of a versatile ES side-population (SP). We aim to compare SP- and non-SP-cells to identify specific characteristics of the SP which points towards a tumor driving functionality of the SP. Due to some stem cell like properties of the SP fraction a comparison to MSCs and normal fibroblasts as a control are also performed.

尤因肉瘤（Ewing's Sarcoma, ES）是一类以存在特定EWS-ETS融合基因为特征的骨恶性肿瘤。本研究选用携带EWSR1-ERG融合基因的模式细胞系CADO-ES1，对多功能尤因肉瘤侧群细胞（side-population, SP）的肿瘤生物学特性进行表征。本研究旨在通过对比侧群细胞与非侧群细胞，明确侧群细胞的特异性特征，以揭示其潜在的肿瘤驱动功能。鉴于侧群组分具备部分干细胞样特性，本研究同时设置间充质干细胞（mesenchymal stem cells, MSCs）与正常成纤维细胞作为对照，开展相关对比实验。