Common and private vitamin D target genes of human primary immune cells: a personalized approach

In this study, the transcriptome of peripheral blood mononuclear cells (PBMCs) of healthy adults was investigated in response to in vitro treatment with 1,25(OH)2D3. Overall design: RNA-seq of PBMCs of 5 individuals (#5, 9,12,13,14) treated in 3 biologial repeats with the biologically active form of vitamin D, 1,25(OH)2D3 (1,25D) or solvent (0.1% Ethanol (EtOH))

本研究探究了健康成人外周血单个核细胞（peripheral blood mononuclear cells，PBMCs）的转录组经体外1,25(OH)2D3处理后的应答情况。整体实验设计：选取5名个体（编号5、9、12、13、14）的PBMCs，分别以维生素D的生物学活性形式1,25(OH)2D3（简称1,25D）或溶剂（0.1%乙醇（EtOH））进行处理，每组设置3次生物学重复，随后对所有样本开展RNA-seq测序。