A SAXS Study on the Nanostructure of Novel Collagen-Stimulating Peptide Amphiphiles

As part of our research programme on self-assembling peptide amphiphiles (PAs) we will investigate the temperature- and pH- dependent self-assembly behaviour of a novel series of PAs based on C16-KTTKS (MatrixylTM) containing the collagen-stimulating KTTKS sequence. BioSAXS will be used to probe the self-assembled structure in aqueous solution, via analysis of form factor in dilute solution. This builds on our earlier research on this system, but the new compounds contain novel lipid chains and PEG spacers desgined to both influence the molecular packing and the bioactivity.

作为我们自组装肽两亲物（PAs）研究计划的一部分，我们将探究一系列基于C16-KTTKS（MatrixylTM）的新型PAs的温度及pH依赖性自组装行为，这些PAs包含具有胶原刺激作用的KTTKS序列。生物小角X射线散射（BioSAXS）将通过分析稀溶液中的形状因子，用于探测水溶液中的自组装结构。本研究建立在我们此前对该体系的研究基础上，但新化合物包含经设计可同时影响分子堆积与生物活性的新型脂质链及聚乙二醇间隔基（PEG spacers）。