The transcription factors SOX9 and SOX5/SOX6 cooperate genome-wide through super-enhancers to drive chondrogenesis (ChIP-Seq)

SOX9 is a transcriptional activator required for chondrogenesis, and SOX5 and SOX6 are closely related DNA-binding proteins that critically enhance its function. We used RNA-seq to charatierize a rat chondrosarcoma (RCS) cells as a faithful model for proliferating/early prehypertrophic growth plate chondrocytes and ChIP-seq to gain novel insights into the full spectrum of the target genes and modes of action of this chondrogenic trio. Overall design: ChIP-seq for SOX9, SOX6 and histone modifications were carried out using RCS cells

SOX9是软骨生成所需的转录激活因子，SOX5与SOX6为与其紧密相关的DNA结合蛋白，可显著增强其功能。本研究采用RNA测序（RNA-seq）对大鼠软骨肉瘤（rat chondrosarcoma, RCS）细胞进行表征，将其作为增殖型/早期前肥大生长板软骨细胞的可靠模型；同时利用染色质免疫共沉淀测序（ChIP-seq）深入解析这一软骨生成三因子的靶基因全貌及作用模式。整体实验设计：以RCS细胞为材料，开展针对SOX9、SOX6以及组蛋白修饰的ChIP-seq实验。