Global gene expression profiles among five independent cytoplasmic male sterile lines.. Oryza sativa

We employed a microarray-based transcriptomic status comparison of rice cytoplasmic male sterile (CMS) lines in order to categorize the nuclear gene expressions upon cytoplasmic substitution. In anthers at the uninucleate and bicellular pollen stages, we found that 8,199 genes significantly changed their expression in at least one of the CMS lines. We categorized the genes into 100 clusters by k-means clustering, and common obvious expression patterns were observed in W11, LD and BT. Overall design: RNA expression was measured for the anthers at uninucleate and bicellular pollen stages of five CMS lines (BT, CW, LD, W11, WA). T65 was used as the wild-type control. Microarray analysis was performed by three replicates using RNA extracted from independent plants.

本研究采用基于微阵列的转录组比较策略，对水稻细胞质雄性不育（CMS）系展开分析，旨在归类细胞质替换条件下的核基因表达模式。在单核与二细胞花粉期的花药样本中，本研究发现共有8199个基因在至少一个CMS系中发生了显著表达变化。本研究通过K均值聚类将这些基因划分为100个表达簇，其中W11、LD与BT三个品系呈现出共通的典型表达模式。
试验设计概述：对5个CMS系（BT、CW、LD、W11、WA）在单核与二细胞花粉期的花药进行RNA表达量检测，以T65作为野生型对照。本研究使用从独立植株中提取的RNA完成三次重复的微阵列分析。