Data for: Alteromonas flava sp. nov. and Alteromonas facilis sp. nov., two novel copper tolerating bacteria isolated from a sea cucumber culture pond in China

Two bacterial strains, P0211T and P0213T, were isolated from a sea cucumber culture pond in China. The strains were able to resist high copper levels. These two strains were characterized at the phenotypic, chemotaxonomic, and genomic level. They were completely different colors, but the 16S rRNA genes showed 99.30% similarity. Phylogenetic analysis based on the sequences of the 16S rRNA gene and five housekeeping genes (dnaK, sucC, rpoB, gyrB, and rpoD) supported the inclusion of these strains within the genus Alteromonas, and the two isolated strains formed a group separated from the closest species Alteromonas aestuariivivens KCTC 52655T. Genomic analyses, including average nucleotide identity (ANIb and ANIm), DNA-DNA hybridization (DDH), and the percentage of conserved proteins (POCP), clearly separated strains P0211T and P0213T from the other species within the genus Alteromonas with values below the thresholds for species delineation. The chemotaxonomic features (including fatty acid and polar lipid analysis) of strains P0211T and P0213T also confirmed their differentiation from the related taxa.
The results demonstrated that strains P0211T and P0213T represented two novel species in the genus Alteromonas, for which we propose the names Alteromonas flava sp. nov., type strain P0211T (= KCTC 62078T = MCCC 1H00242T), and Alteromonas facilis sp. nov., type strain P0213T (= KCTC 62079T = MCCC 1H00243T).

两株细菌菌株P0211^T与P0213^T分离自中国的一处海参养殖池塘，二者可耐受高浓度铜离子环境。本研究从表型、化学分类学及基因组三个层面完成了对这两株菌株的分类学表征：二者菌落颜色完全不同，但16S核糖体RNA基因（16S rRNA gene）序列相似性仅为99.30%。基于16S rRNA基因及5个持家基因（dnaK、sucC、rpoB、gyrB及rpoD）序列的系统发育分析结果显示，两菌株隶属于交替单胞菌属（Alteromonas），且二者共同构成一个独立的进化类群，与亲缘关系最近的物种Alteromonas aestuariivivens KCTC 52655^T相互分离。基因组分析包括平均核苷酸同源性（ANIb与ANIm）、DNA-DNA杂交（DDH）及保守蛋白百分比（POCP），分析结果表明，两菌株P0211^T与P0213^T与交替单胞菌属内其他物种的各项指标值均低于物种界定阈值，证实二者与其他物种存在显著分化。两菌株的化学分类学特征（涵盖脂肪酸与极性脂组分分析）同样佐证了其与近缘类群的区分性。

本研究结果证实，菌株P0211^T与P0213^T为交替单胞菌属内的两个新物种，现将其分别命名为黄色交替单胞菌新种（Alteromonas flava sp. nov.），模式菌株为P0211^T（= KCTC 62078^T = MCCC 1H00242^T），以及易生交替单胞菌新种（Alteromonas facilis sp. nov.），模式菌株为P0213^T（= KCTC 62079^T = MCCC 1H00243^T）。