Gene expression profiles in maspin-overexpressing RERF-LC-AI and LK-2 lung squamous cell carcinoma cell lines

To investigate the subcellular localization of maspin in lung squamous cell carcinoma (LUSC) and its functional relevance, gene expression profiles were analyzed in RERF-LC-AI and LK-2 cells overexpressing maspin. The data provides insight into the differential function of maspin in LUSC depending on its subcellular localization. Overall design: Establishment of control and maspin-overexpressing cell lines using RERF-LC-AI and LK-2 cells was performed by transduction with lentiviral vectors. Total RNA from these cells were recovered using Rneasy MINI Kit. To purify the strand-specific mRNA library, the mRNA was reverse transcribed into cDNA using random primers, and then the synthesized double-strand cDNA was fragmented, and the cDNA was subsequently ligated adapters. Transcriptome sequencing in strand-specific mRNA-seq library was performed using illumine NovaSeq 6000 Sequencing System.

为探讨maspin在肺鳞状细胞癌（lung squamous cell carcinoma, LUSC）中的亚细胞定位及其功能相关性，本研究对过表达maspin的RERF-LC-AI与LK-2细胞开展了基因表达谱分析。本数据集可为阐明maspin在肺鳞状细胞癌中依赖其亚细胞定位的差异功能提供研究依据。
整体实验设计：通过慢病毒载体转染，分别构建RERF-LC-AI及LK-2细胞的对照组与maspin过表达细胞系。采用RNeasy MINI试剂盒提取上述细胞的总RNA。为纯化链特异性mRNA文库，先以随机引物将mRNA反转录为cDNA，随后将合成的双链cDNA进行片段化处理并连接测序接头。采用Illumina NovaSeq 6000测序系统完成链特异性mRNA-seq文库的转录组测序。