T-DNA insertion sites in C. higginsianum ATMT mutants.
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1 Number of T-DNAs insertions as determined by Southern Blot analysis.2 Position of the T-DNA border sequence on Colletotrichum Database Supercontigs (Colletotrichum Sequencing Project, Broad Institute of Harvard and MIT, http://www.broadinstitute.org/) or on Colletotrichum higginsianum Database Contigs (Max Planck Institute for Plant Breeding Research, http://gbrowse.mpiz-koeln.mpg.de/cgi-bin/gbrowse/colletotrichum_higginsianum_public/). The sequenced border sequence is given as left border (LB) or right border (RB).3 Gene IDs from Colletotrichum Databases (see 2); upstream: distance to start codon, downstream: distance to stop codon, ORF: in open reading frame.4 Best BLAST hit against NCBI non-redundant protein sequences database (hypothetical and C. higginsianum proteins excluded).T-DNA insertion sites in C. higginsianum ATMT mutants.
1. 通过Southern印迹(Southern Blot)分析得到的T-DNA(Transfer DNA)插入拷贝数目。 2. T-DNA边界序列在炭疽菌属(Colletotrichum)数据库超级重叠群(Colletotrichum测序项目,哈佛与MIT博德研究所,http://www.broadinstitute.org/)或哈氏炭疽菌(Colletotrichum higginsianum)数据库重叠群(马克斯·普朗克植物育种研究所,http://gbrowse.mpiz-koeln.mpg.de/cgi-bin/gbrowse/colletotrichum_higginsianum_public/)上的定位信息。所测序获得的边界序列标注为左边界(Left Border, LB)或右边界(Right Border, RB)。 3. 来源于炭疽菌属数据库的基因ID(参见第2条);上游区域:距起始密码子的距离,下游区域:距终止密码子的距离,开放阅读框(Open Reading Frame, ORF):序列位于开放阅读框内。 4. 针对美国国家生物技术信息中心(National Center for Biotechnology Information, NCBI)非冗余蛋白质序列数据库的最优BLAST(Basic Local Alignment Search Tool)比对结果(已排除假设蛋白及哈氏炭疽菌蛋白质序列)。 本数据集收录哈氏炭疽菌ATMT(Agrobacterium tumefaciens-mediated transformation,农杆菌介导的转化)突变体的T-DNA插入位点相关信息。



