METH consumption induced the differential expression of miRNA in Rat model

Male rats received daily injections of saline or methamphetamine (10 mg/kg, at 12 h intervals) for 2 weeks and for 4 weeks. Changes in gene expression were assessed by miRNA-sequencing after 6 hours following the last injection. Methamphetamine induced changes in the whole transcriptome were significantly greater in the left ventricle of the hearts than that in the normal rat both in terms of the number of genes affected and the magnitude of the changes. Overall design: Gene expression profiling of the left ventricle tissue of rats following treatment with methamphetamine or saline.

雄性大鼠每12小时接受一次生理盐水或甲基苯丙胺（methamphetamine，10 mg/kg）注射，分别持续2周与4周。末次给药6小时后，通过miRNA测序（miRNA-sequencing）评估基因表达变化。与正常大鼠相比，甲基苯丙胺诱导的全转录组变化在大鼠心脏左心室中更为显著，无论是受影响基因的数量还是变化幅度均是如此。实验整体设计：对经甲基苯丙胺或生理盐水处理后的大鼠左心室组织开展基因表达谱分析。