ATAC-seq data from Lmna knock-out mouse embryonic fibroblasts (Lmna-/- MEFs) and wild type (Lmna+/+ MEFs)

Using ATAC seq analysis, we showed that the MEFs with a knockout of Lmna gene (i.e., missing the lamin A/C nuclear scaffolding protein) (Lmna-/- MEFs) display a striking change in chromatin accessibility landscape (peak signals that are both up and down), both within and outside lamina-associated domains (LADs); moreover, there was a clear overrepresentation of peaks with a gain in chromatin accessibility (within and outside LADs) in the Lmna-/- MEFs, and within LADs compared to outside LADs. Overall design: ATAC-seq of four biological replicates of mouse embryonic fibroblasts (MEFs) with the genotypes Lmna-/- and Lmna+/+.

本研究借助转座酶可及性染色质测序（ATAC-seq）分析，证实Lmna基因敲除的小鼠胚胎成纤维细胞（即缺失核骨架蛋白层粘连蛋白A/C的Lmna-/- MEFs）的染色质可及性图谱呈现显著改变——既存在上调的峰信号，也存在下调的峰信号，该改变同时发生于层粘连蛋白相关结构域（LADs）的内部与外部；进一步分析显示，Lmna-/- MEFs中染色质可及性升高的峰（在LADs内外均有分布）存在明显富集，且相较于LADs外部，LADs内部的此类峰富集更为显著。整体实验设计：对基因型为Lmna-/-与Lmna+/+的小鼠胚胎成纤维细胞（mouse embryonic fibroblasts, MEFs）各开展4次生物学重复的ATAC-seq测序。