Research Note: Possible influence of thermal selection on patterns of HSP70 and HSP90 gene polymorphisms in Thai indigenous and local chicken breeds and red junglefowls

The thermal stress caused by global climate change adversely affects the welfare, productivity, and reproductive performance of farm animals, including chickens, and causes substantial economic losses. However, the understanding of the genetic basis of the indigenous chicken adaptation to high ambient temperatures is limited. Hence, to reveal the genetic basis of thermal stress adaptation in chickens, this study investigated polymorphisms in the heat shock protein 70 (HSP70) and HSP90 genes, known mechanisms of cellular defense against thermal stress in indigenous and local chicken breeds and red junglefowls in Thailand. The result revealed seven alleles of the HSP70 gene. One allele exhibited a missense mutation, where an amino acid changed from Asn to His in the substrate-binding and peptide-binding domains, which is exclusive to the Lao Pa Koi chicken breed. Twenty new alleles with silent mutations in the HSP90 gene highlighted its greater complexity. Despite this diversity, distinct..., This data has been collected from the Illumina paired-end short-read sequencing. The raw sequences were then demultiplexed and processed by the AMPLISAS website to allele genotyping purposes.   , , # Data from: Research Note: Possible influence of thermal selection on patterns of HSP70 and HSP90 gene polymorphisms in Thai indigenous and local chicken breeds and red junglefowls

[https://doi.org/10.5061/dryad.tmpg4f54h](https://doi.org/10.5061/dryad.tmpg4f54h) 

## Description of the data and file structure

This is a genotyping data of the HSP70 and HSP90 alleles in the Thai indigenous chicken breeds and red junglefowl. (HSP70_data.csv, HSP90_data.csv)

This dataset also contained figures, tables, and supplementary tables and figures uploaded to Zenodo as Supplemental Information. 

## Sharing/Access information

Allele sequences found in this study were deposited in the DNA Data Bank of Japan ([https://www.ddbj.nig.ac.jp/](https://www.ddbj.nig.ac.jp/), accessed on August 19, 2023) (accession numbers: LC775531– LC775537 and LC775538– LC775557).

## Code/Software

N/a
,

全球气候变化引发的热应激，会对包括家鸡在内的农场畜禽的福利、生产性能与繁殖表现产生不利影响，并造成显著的经济损失。然而，目前学界对本土鸡适应高温环境的遗传基础认知仍较为有限。为此，本研究针对热休克蛋白70（HSP70）与热休克蛋白90（HSP90）基因的多态性展开探究——这两类基因是已知的细胞抵御热应激的关键调控因子，研究对象涵盖泰国本土及地方鸡品种与红原鸡。
研究结果显示，HSP70基因共存在7个等位基因。其中1个等位基因携带错义突变：其底物结合域与肽结合域内的氨基酸由天冬酰胺（Asn）突变为组氨酸（His），该突变仅存在于老挝巴科伊（Lao Pa Koi）鸡品种中。HSP90基因中共发现20个携带沉默突变的新等位基因，凸显了该基因的更高复杂性。尽管存在上述多样性，但存在显著差异……
本研究的数据通过Illumina双端短读长测序获取。原始序列经解多路复用后，通过AMPLISAS网站完成等位基因分型处理。

# 数据源自：研究简报：热选择对泰国本土及地方鸡品种与红原鸡的HSP70和HSP90基因多态性模式的潜在影响
https://doi.org/10.5061/dryad.tmpg4f54h

## 数据与文件结构说明
本数据集包含泰国本土鸡品种与红原鸡的HSP70及HSP90等位基因分型数据（HSP70_data.csv、HSP90_data.csv）。
此外，本数据集还包含以补充材料形式上传至Zenodo平台的相关图件、表格及补充表格与图件。

## 共享与获取途径
本研究发现的等位基因序列已提交至日本DNA数据库（DDBJ，https://www.ddbj.nig.ac.jp/，2023年8月19日访问），登录号为LC775531—LC775537与LC775538—LC775557。

## 代码与软件
无（N/a）