Caspase 3 activity in patients with VWD-type 2B.

Washed platelets (2.5 x108 platelets/mL) pretreated or not with ABT-737 (10 μM) were lysed and caspase-3 activity was assessed by immunoblotting with A) an anti-caspase-3 antibody B) with an anti-gelsolin antibody and C) with anti-calpain 1 antibody. Results are representative of two or three independent experiments.

将经或未经ABT-737（10 μM）预处理的洗涤血小板（2.5×10⁸个血小板/毫升）裂解后，采用免疫印迹法检测半胱天冬酶-3（caspase-3）活性，检测时分别使用A）抗半胱天冬酶-3抗体、B）抗凝溶胶蛋白抗体以及C）抗钙蛋白酶1抗体。实验结果均为2~3次独立重复实验的代表性结果。