Switching of OAS1 splicing isoforms mitigates SARS-CoV-2 infection [Caco-2]

We aimed to study impact of CDC-like kinase (CLK) inhibitor CaNDY on OAS1 splicing manipulation. Our data indicates CaNDY treatment induces switching of OAS1 splice isoforms to provide an increased resistance to SARS-CoV-2 infection. PolyA-tailed RNA profiles were obtained for Caco-2 cells, treated with 10 µM CaNDY (n=3) or 0.1% DMSO (n=3).

我们旨在探究CDC样激酶（CDC-like kinase, CLK）抑制剂CaNDY对2'-5'-寡腺苷酸合成酶1（OAS1）剪接调控的影响。本研究数据显示，CaNDY处理可诱导OAS1剪接异构体的转换，进而提升细胞对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的感染抗性。我们获取了分别经10 μM CaNDY（n=3）或0.1%二甲基亚砜（DMSO，n=3）处理的Caco-2细胞的polyA尾RNA表达谱数据。