Expression data from SPARKS CHARMS JIA cohort. Homo sapiens

Gene expression on peripheral blood mononuclear cells (PBMC) from SPARKS CHARMS juvenile idiopathic arthritis (JIA) cohort pre and post methotrexate therapy. This is the first study to our knowledge, to evaluate gene expression profiles in children with JIA before and after MTX, and to analyze genetic variation in differentially expressed genes. We have identified a gene, which may contribute to genetic variability in MTX response in JIA. Overall design: PBMC were isolated from blood samples taken from 11 patients with JIA pre methotrexate therapy and at 6 months into therapy

本数据集涵盖SPARKS CHARMS幼年特发性关节炎（juvenile idiopathic arthritis, JIA）队列中，接受甲氨蝶呤（methotrexate, MTX）治疗前后的外周血单个核细胞（peripheral blood mononuclear cells, PBMC）基因表达谱数据。
据我们所知，本研究为首例评估JIA儿童患者甲氨蝶呤治疗前后基因表达谱，并分析差异表达基因（differentially expressed genes）遗传变异的研究。本团队已鉴定出一个可能与JIA患者甲氨蝶呤治疗应答的遗传异质性相关的基因。
总体设计：本研究从11名JIA患者体内采集了甲氨蝶呤治疗前及治疗6个月时的血液样本，并从中分离得到外周血单个核细胞。