Transcription activated by AP2-Z in early zygote plays an essential role in Plasmodium ookinete development [RNA-seq]

Plasmodium berghei transcriptomes of wild-type and ap2-z knockout parasites [ap2-z(-)] were analyzed at 6 hours post starting ookinete culture (hpoc). Overall design: Wild-type and ap2-z(-) were harvested at 6 hpoc, and total RNA was extracted. Libraries were constructed from the RNA and then sequenced by the Next Generation Sequencing.

本研究针对动合子培养起始后6小时（hpoc）的野生型与ap2-z基因敲除（ap2-z(-)）伯氏疟原虫（Plasmodium berghei）的转录组展开分析。实验整体设计如下：于6 hpoc时收集野生型与ap2-z(-)疟原虫，提取总RNA，以该总RNA构建测序文库，随后通过下一代测序（Next Generation Sequencing）完成测序。