AFLP_Based_70Gb以上大基因组优化实验数据
收藏海数据2026-05-02 收录
下载链接：
https://haidatas.com/dataset/aflp_based_70gbyishangdajiyinzuyouhuashiya_72a3c351
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资源简介：
本数据集展示了针对70Gb以上超大基因组的AFLP实验优化方案，使用识别8碱基序列的限制性内切酶（AscI和SbfI），通过+3选择性碱基引物减少扩增片段数量，同时分析片段数量与基因组大小对同源片段（大小同塑性）的影响，以及不同反应条件对槲寄生等超大基因组物种实验重复性和条带强度的作用。 论文“Optimisation of AFLP for extremely large genomes over 70 Gb”
This dataset presents an optimized AFLP experimental protocol for extremely large genomes exceeding 70 Gb. It uses restriction endonucleases AscI and SbfI that recognize 8-bp recognition sequences, employs primers with 3 selective bases to reduce the number of amplified fragments, and analyzes the effects of fragment quantity and genome size on homologous fragments (size homoplasy), as well as the impacts of different reaction conditions on experimental repeatability and band intensity in extremely large genome species such as mistletoe. The associated paper is titled "Optimisation of AFLP for extremely large genomes over 70 Gb"
提供机构：
公开数据
创建时间：
2026-01-30
