Dynamic single-cell RNA sequencing identifies immunotherapy persister cells following PD-1 blockade. Dynamic single-cell RNA sequencing identifies immunotherapy persister cells following PD-1 blockade

We report results of transcriptional profiling of ex vivo MC38 murine organotypic tumor spheroids following programmed death-1 (PD-1) blockade compared to isotype control IgG treatment by bulk and single-cell RNA-sequencing after 6 days of treatment. Additionally, we report results from single-cell RNA-sequencing of MC38 tumors treated in vivo with PD-1 blockade versus isotype control IgG to validate ex vivo workflow. Overall design: Transcriptional profiling of residual MC38 tumor cells after PD-1 blockade

本研究报道了经6天处理后，通过批量RNA测序（bulk RNA-sequencing）与单细胞RNA测序（single-cell RNA-sequencing），对比同型对照IgG（isotype control IgG）治疗组，经程序性死亡蛋白1（programmed death-1, PD-1）阻断处理后的离体（ex vivo）MC38小鼠器官型肿瘤球体的转录组谱分析结果。
此外，本研究还报道了MC38小鼠体内（in vivo）肿瘤经程序性死亡蛋白1阻断剂与同型对照IgG处理后的单细胞RNA测序结果，以验证离体实验流程的可靠性。
整体实验设计：程序性死亡蛋白1阻断处理后残留的MC38肿瘤细胞的转录组谱分析。