Cav3.2-/- Mouse #336 CA1 EEG recording

A Cav3.2-/- mouse (internal #336) was implanted with an intrahippocampal electrode for electrohippocampal CA1 EEG recordings. The differential electrode of the TA10ETA-F20 transmitter (Data Science International, DSI, USA, technical specifications: weight 3.9 g, volume 1.9 cc, input voltage range ± 2.5 mV, channel bandwidth (B) 1–200 Hz, nominal sampling rate (f) 1000 Hz (f = 5 B), temperature operating range 34–41 °C) was positioned at the following stereotaxic coordinates: (+)-lead, caudal − 2 mm, lateral of bregma 1.5 mm (right hemisphere), and dorsoventral (depth) 1.5 mm. The epidural reference electrode was positioned on the surface of the cerebellar cortex at the following stereotaxic coordinates: (−)-lead, bregma − 6 mm and lateral of bregma 1 mm (right<br> hemisphere). The deep tungsten electrodes (FHC, USA) are encapsuled with epoxylite with an<br> impedance of 50–100 kΩ (measured at 1000 Hz) and a shank diameter of 250 μm. For further details on the Cav3.2 mouse model, animal experimentation approval, anaesthesia, transmitter insertion, stereotaxic electrode implantation, postoperative pain management and recovery, please refer to Arshaad MI et al., 2021. EEG data were exported to NeuroScore 3.2.9306-1 (Data Sciences International, DSI, USA). As the NeuroScore file format does not allow free access to the data, we have exported the raw EEG data as asci.-files, so that they can easily be imported in secondary analysis software. Importantly, the exported EEG data represent raw EEG data. No filtering, no artefact detection and/or artefact removal have been carried out. Thus, it is the users responsibility to adequately pretreat the data before further processing and analysis. As described in Arshaad MI et al. (2021), filtering, artefact detection and removal was carried out using NeuroScore before further analysis. The EEG data are presented here as a zip-folder for size reasons. Four EEG recordings are exported: R1 (a first 24h long-term EEG recording 10 days post transmitter implantation), R2 (a second 24h long-term recording 17 days post transmitter implantation), U1 (a 6h EEG recording following the first urethane injection (800 mg/kg i.p.) at day 18 post transmitter implantation), U2 (a 6h EEG recording after a second urethane injection (800 mg/kg i.p.) at day 25 post transmitter implantation). In addition, relative activity values are exported for all four recordings. For details on the recording procedure, please refer to Arshaad MI et al. (2021).本数据集采用编号为#336的Cav3.2基因敲除（Cav3.2-/-）小鼠，于其海马内植入电极以开展海马CA1区脑电图（electroencephalogram, EEG）记录。本次实验所用的TA10ETA-F20型发射器（美国Data Science International公司，简称DSI）的差分电极按以下立体定位坐标植入：正极引线相对于前囟（bregma）尾侧2 mm、右侧半球旁开1.5 mm，背腹侧（深度）1.5 mm，该发射器技术参数如下：重量3.9 g，体积1.9 立方厘米，输入电压范围±2.5 mV，通道带宽（B）1–200 Hz，标称采样率（f）1000 Hz（f=5B），工作温度范围34–41 ℃。硬膜外参考电极置于小脑皮质表面，定位坐标为：负极引线相对于前囟尾侧6 mm、右侧半球旁开1 mm。深部钨电极（美国FHC公司）采用epoxylite环氧包封材料封装，阻抗为50–100 kΩ（于1000 Hz下测得），电极柄直径250 μm。关于Cav3.2基因敲除小鼠模型、动物实验伦理审批、麻醉操作、发射器植入、立体定位电极植入、术后镇痛与恢复的详细信息，请参阅Arshaad MI等人2021年的研究成果。脑电图数据先导出至NeuroScore 3.2.9306-1软件（美国Data Science International公司，简称DSI），由于NeuroScore软件的文件格式无法直接开放数据访问，我们将原始脑电图数据导出为ASCII文件（原文标注为asci.-files，应为笔误），以便于在二次分析软件中直接导入。需特别说明的是，本次导出的脑电图数据均为原始数据，未经过滤波、伪迹检测或伪迹去除处理，因此用户需在后续处理与分析前对数据进行恰当的预处理。如Arshaad MI等人（2021）的研究所述，在开展进一步分析前，需使用NeuroScore软件完成滤波、伪迹检测与去除操作。考虑到文件大小，本次公开的脑电图数据以ZIP压缩包形式提供。本次共导出4组脑电图记录：R1（发射器植入后第10天的首次24小时长期脑电图记录）、R2（发射器植入后第17天的第二次24小时长期脑电图记录）、U1（发射器植入后第18天，首次乌拉坦（urethane）腹腔注射（intraperitoneal, i.p.）800 mg/kg后的6小时脑电图记录）、U2（发射器植入后第25天，第二次乌拉坦腹腔注射800 mg/kg后的6小时脑电图记录）。此外，我们还导出了4组记录对应的相对活动度数值。关于记录流程的详细信息，请参阅Arshaad MI等人2021年的研究成果。