Nitrogen cycling in the Lake Lugano North Basin & isotope effects during enzymatic nitrate reduction

The dataset accompanies the manuscripts "Microbial nitrogen removal versus recycling in the redox transition zone of a meromictic lake and its coupling to sulfur” and “Nitrogen and oxygen isotope effects during enzymatic nitrate reduction in vitro and by natural lake water consortia” Files: 16S rRNA gene sequencing data from the Lake Lugano North Basin and Lake La Cruz: - Mapping file - ASV table - Refseq file -Tree file - R Skript data import - R Skript relative abundance analysis - R Skript alpha and beta diversity analysis Isotope effects during nitrate reduction in incubation experiments with Escherichia coli and Pseudomonas sp. strains harboring narG and/or napA nitrate reductase genes: - Raw data, parameters (N-species concentrations and nitrate isotopes over time) - Raw data, values (N-species concentrations and nitrate isotopes over time) - Isotope effects, parameters (Nitrate reduction and nitrite production rates, Rayleigh isotope effects, Δδ18O:Δδ15N-NO3- ratios) - Isotope effects, values (Nitrate reduction and nitrite production rates, Rayleigh isotope effects, Δδ18O:Δδ15N-NO3- ratios)本数据集配套两篇学术手稿，分别为《分层湖泊（meromictic lake）氧化还原过渡带的微生物脱氮与氮回收机制及其与硫循环的耦合作用》与《体外酶促硝酸盐还原及天然湖水菌群（natural lake water consortia）的氮氧同位素分馏效应》。 数据文件： 1. 卢加诺湖北盆地（Lake Lugano North Basin）与拉克鲁兹湖（Lake La Cruz）的16S rRNA基因测序数据： - 映射文件 - ASV（扩增子序列变异）表 - 参考序列文件 - 系统发育树文件 - 数据导入用R脚本 - 相对丰度分析用R脚本 - Alpha与Beta多样性分析用R脚本 2. 携带narG和/或napA硝酸盐还原酶基因的大肠杆菌（Escherichia coli）与假单胞菌属（Pseudomonas sp.）菌株的培养实验中，硝酸盐还原过程的同位素分馏效应相关数据： - 原始数据与参数（随时间变化的氮物种浓度及硝酸盐同位素比值） - 原始数据与数值（随时间变化的氮物种浓度及硝酸盐同位素比值） - 同位素分馏参数（硝酸盐还原速率、亚硝酸盐生成速率、瑞利同位素分馏效应、Δδ¹⁸O:Δδ¹⁵N-NO₃⁻比值） - 同位素分馏数值（硝酸盐还原速率、亚硝酸盐生成速率、瑞利同位素分馏效应、Δδ¹⁸O:Δδ¹⁵N-NO₃⁻比值）