Expansion of Tregs and prevention of pathological neuroinflammation following local delivery of IL2

Supplementary Resource 1. High resolution Treg localization in coronal sections of wildtype and αCamKIIIL2 mice. Healthy perfused mouse brains from wildtype and αCamKIIIL2 mice were compared by immunofluorescent confocal imaging. CD4 (green), Foxp3 (red), laminin α4 (vascular basement membrane, white) and DAPI (blue). Left, wildtype mouse. Right, αCamKIIIL2 mouse. High resolution allows zooming and panning for localization of Tregs across the entire coronal section. Supplementary Resource 2. Code for single-cell RNA-seq analysis for wildtype vs αCamKIIIL2 mice. Code is provided in .pdf and .Rmd formats. Supplementary Resource 3. Code for single-cell RNA-seq analysis for PHP.GFAP-GFP- vs PHP.GFAP-IL2-treated mice. Code is provided in .pdf and .Rmd formats.

补充材料1. 野生型（wildtype）与αCamKIIIL2小鼠冠状切片中调节性T细胞（Treg）的高分辨率定位分析。本研究通过免疫荧光共聚焦成像技术，对比分析了野生型与αCamKIIIL2小鼠经健康灌注处理后的脑组织标本。荧光标记包括：CD4（绿色）、Foxp3（红色）、层粘连蛋白α4（血管基底膜，白色）以及DAPI（蓝色）。图像左侧为野生型小鼠样本，右侧为αCamKIIIL2小鼠样本。该高分辨率图像支持缩放与平移操作，可实现全冠状切片范围内调节性T细胞的精准定位观察。
补充材料2. 野生型与αCamKIIIL2小鼠单细胞RNA测序（single-cell RNA-seq）分析代码。本代码以.pdf和.Rmd格式提供。
补充材料3. PHP.GFAP-GFP-与PHP.GFAP-IL2处理小鼠的单细胞RNA测序分析代码。本代码以.pdf和.Rmd格式提供。