Flow cytometry dataset of dexamethasone and immune checkpoint blockade in 4T1 breast cancer model (Ki67 and FoxP3)

Flow cytometry analyses were conducted on cells isolated from tumors and draining lymph nodes (dLNs) of 4T1-bearing mice to assess lymphocyte proliferation. Ki67 staining was used as a proliferation marker together with surface and intracellular markers to define T cell subsets. Cells were stained with Zombie Aqua for viability, PE–Foxp3, PE-CF594–conjugated F4/80, Ly6G, and B220 (dump channel), PerCP-Cy5.5–CD4, APC/Cy7–CD8, Pacific Blue–CD45, and PE/Cy7–Ki67. Tumor-infiltrating lymphocytes and dLN-resident T cells were gated and analyzed for Ki67 expression under dexamethasone, immune checkpoint blockade (ICB), or combination treatment conditions. These data provide insights into how dexamethasone influences the proliferative capacity of T cells in both the tumor microenvironment and secondary lymphoid tissues.本研究针对4T1荷瘤小鼠的肿瘤组织及引流淋巴结（draining lymph nodes，dLNs）中分离得到的细胞开展流式细胞术（flow cytometry）分析，以评估淋巴细胞增殖情况。本研究以Ki67染色作为增殖标志物，联合表面及胞内标志物对T细胞亚群进行定义。实验细胞经以下试剂染色：用于活力检测的Zombie Aqua染料、PE标记的Foxp3、PE-CF594偶联的F4/80、Ly6G与B220（排除通道（dump channel））、PerCP-Cy5.5标记的CD4、APC/Cy7标记的CD8、Pacific Blue标记的CD45，以及PE/Cy7标记的Ki67。研究对肿瘤浸润淋巴细胞（tumor-infiltrating lymphocytes）及引流淋巴结驻留T细胞进行设门分析，检测地塞米松、免疫检查点阻断（immune checkpoint blockade，ICB）或联合处理条件下的Ki67表达水平。本数据集可为阐明地塞米松如何影响肿瘤微环境与次级淋巴组织中T细胞的增殖能力提供研究依据。