Hi-C seq of cardiomyocyte nuclei of H2BmCh iOE mouse. Mus musculus strain:ICR

Cardiomyocyte nuclei are constantly exposed to mechanical stress. It remains mysterious how they maintain nuclear shape throughout a life. We found that high-level overexpression of H2B-mCherry in mouse cardiomyocytes causes elongation of nuclei with disrupted spatial organizations of heterochromatin at microscopic level. Cardiomyocyte nuclei in H2B-mCherry iOE mice show heterochromatin disruption at 2 weeks after TMX induction, leading to nuclear deformation, resulting in fatal heart failure at 6 weeks after TMX induction. The purpose of this Hi-C seq is to investigate the change of higher order genomic structures accompanied with heterochromatin disruption. We isolated cardiomyocyte nuclei from H2B-mCherry iOE mouse hearts at 0 days and 2 weeks post-TMX with FACS, subjected to library construction for Hi-C seq.

心肌细胞核持续承受机械应力。目前学界尚未明确它们如何在整个生命周期中维持细胞核形态。我们发现，在小鼠心肌细胞中高表达H2B-mCherry会导致细胞核伸长，并在微观层面破坏异染色质（heterochromatin）的空间排布。在经他莫昔芬（TMX）诱导2周后，H2B-mCherry诱导型过表达（iOE）小鼠的心肌细胞核会出现异染色质紊乱，进而引发细胞核变形，并最终于TMX诱导后6周导致致命性心力衰竭。本Hi-C测序（Hi-C seq）旨在探究伴随异染色质紊乱发生的高阶基因组结构变化。我们通过荧光激活细胞分选术（FACS），从TMX处理后0天及2周的H2B-mCherry iOE小鼠心脏中分离心肌细胞核，随后进行Hi-C测序的文库构建。