GuHCl-induced unfolding of L35Ae samples
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GuHCl-induced unfolding of L35Ae samples followed by fluorescence intensity at 314 nm and position of tryptophan fluorescence spectrum maximum. Excitation wavelength was 280 nm. Protein concentration was 3 µM. Buffer conditions: PBS, 150 mM NaCl, pH 7.0. Boltzmann function was used for theoretical fits of the experimental data.
以314 nm处的荧光强度与色氨酸荧光光谱峰值位置为检测指标，跟踪盐酸胍（GuHCl）诱导的L35Ae样品解折叠过程。激发波长设定为280 nm，蛋白质浓度为3 µM。缓冲液条件为：磷酸盐缓冲液（PBS）、150 mM氯化钠，pH 7.0。实验数据的理论拟合采用玻尔兹曼函数完成。
创建时间：
2015-06-29
