Distribution of pineal RNA sequencing reads mapping for Y RNA regions.

Short reads aligning to the Y RNA region are divided into three groups. “Total # of reads at location” indicates how many reads were aligned to each Y RNA region. “Number of locations” provides a breakdown of the total numbers – reporting genome-wide multiple mapping characteristics: of the three bins; “1” - uniquely mapped, “2 to 5” – mapping 2,3,4 or 5 times and “6 to 10” – mapping 6,7,8,9 or 10 times to the rat genome. These same figures are calculated as a percentage – “Percent of reads at location” - and show that pY RNA1-s2 contains 98% uniquely mapped reads.

比对至Y RNA区域的短读长序列（short reads）被划分为三组。"位点总序列数（Total # of reads at location）"表示比对至每个Y RNA区域的序列总数。"位点数（Number of locations）"用于对总序列数进行细分，以呈现全基因组范围内的多比对特征：分为三类分箱，其中"1"代表唯一比对，"2至5"代表比对2、3、4或5次，"6至10"代表比对6、7、8、9或10次至大鼠基因组。上述统计值同样以百分比形式计算得到，即"位点序列占比（Percent of reads at location）"，结果显示pY RNA1-s2的唯一比对序列占比达98%。