Tbet vs Eomes [RNA-Seq]

T-bet and Eomes are related T-box transcription factors that control NK cell development. This study was designed to understand the specific roles of Eomes and T-bet in regulating gene expression. RNAseq data were generated for immature (CD11b- CD27+) and mature (CD11b+ CD27-) NK cells from T-bet KO (Tbet Ho) or control mice (Tbet WT) or from Eomes KO (Eomes Ho) or control mice (NK-Cre). Three samples were generated for each condition (Tri 1, 2, 3). Overall design: Three biological replicates for each condition were performed (ie three different flow cytometry sorts for each genotype and for each maturation stage).

T-bet与Eomes是调控自然杀伤（NK）细胞发育的相关T-box转录因子（T-box transcription factors）。本研究旨在阐明Eomes与T-bet在基因表达调控中的特异性功能。本研究针对源自T-bet基因敲除（T-bet KO，Tbet Ho）或对照小鼠（Tbet WT）、以及Eomes基因敲除（Eomes KO，Eomes Ho）或对照小鼠（NK-Cre）的未成熟（CD11b- CD27+）与成熟（CD11b+ CD27-）自然杀伤细胞，生成了RNA测序（RNA-seq）数据。每种实验条件均生成3份样本（Tri 1、2、3）。实验整体设计：每种实验条件均设置3次生物学重复，即针对每种基因型与每个成熟阶段，分别开展3次独立的流式细胞术分选。