Assessment of population nAb detection using reassortant pp.

aFor the hemagglutination inhibition test, the titers of the virus and pp were adjusted to 4 hemagglutination units.bFor the microneutralization assays, the titers of the virus and pp were adjusted to 100 TCID50. The 95% inhibitory concentration (IC95) was used for nAb analysis.Assessment of population nAb detection using reassortant pp.

a、 血凝抑制试验（hemagglutination inhibition test）中，将病毒与假病毒颗粒（pseudoparticles，pp）的效价校准至4个血凝单位。b、 微量中和试验（microneutralization assays）中，将病毒与假病毒颗粒的效价校准至100 TCID₅₀（50%组织细胞感染量）；以95%抑制浓度（IC95）作为中和抗体（neutralizing antibody，nAb）分析的判定依据。采用重配假病毒颗粒（reassortant pp）开展人群中和抗体检测的性能评估。