Additional Supplement Data of the manuscript "Epigenome-Wide Association Study of Asthma Exacerbations in Europeans"

For this manuscript we conducted an epigenome-wide association study of asthma exacerbations following a discovery and validation design. The discovery phase includes GEMAS study, which is divided into two subsets (312 Canary Islanders and 94 Basques). Separately summary statistics are avaliable for each subset:  "25_EWAS_GEMAS_EPIC_Exacerbations_mval_lineal_age-sex-batch-pcs-ccReFACTor_filtered.txt" for Canary Islanders and "25_EWAS_GEMAS_PV_EPIC_Exacerbations_mval_lineal_age-sex-batch-pcs-ccReFACTor_filtered.txt" for Basques including the following columns:

probes: Probe ID

logFC:  estimate of the log2-fold-change corresponding to the effect

SE: standard error

CI.L:  left limit of confidence interval for logFC

CI.R:  right limit of confidence interval for logFC

t: moderated t-statistic

P.Value: raw p-value

adj.P.Val: adjusted p-value by false discovery rate

CHR: chromosome

pos: genomic position based on hg38/GRCh38

UCSC_RefGene_Name: gene annotation based on Illumina EPIC Manifest file v 1.0 B4

In addition, we performed a meta-analysis whose results summary statistics output is organized in the following columns:

RSID: Probe ID

STUDY: number of studies or subsets were is present the probe

BETA_meta:  coefficient estimate

SEBETA_meta:  standard error

PVALUE_meta: raw p-value 

PVALUE_Q: p-value of Cochran’s Q

Model: FE (fixed effect) or RE2 (random effect)

PVALUE_meta_adj: adjusted p-value by false discovery rate

本研究采用发现-验证研究设计，开展了哮喘急性加重的全表观基因组关联研究（epigenome-wide association study, EWAS）。发现阶段纳入GEMAS研究队列，该队列分为两个亚组：312名加那利群岛居民与94名巴斯克人。两个亚组均分别提供了汇总统计数据：针对加那利群岛居民的文件为「25_EWAS_GEMAS_EPIC_Exacerbations_mval_lineal_age-sex-batch-pcs-ccReFACTor_filtered.txt」，针对巴斯克人的文件为「25_EWAS_GEMAS_PV_EPIC_Exacerbations_mval_lineal_age-sex-batch-pcs-ccReFACTor_filtered.txt」，其包含以下列字段：
1. 探针ID（Probe ID）
2. logFC：对应效应的log2倍变化估计值
3. SE：标准误（standard error）
4. CI.L：logFC置信区间下限
5. CI.R：logFC置信区间上限
6. t：校正t统计量（moderated t-statistic）
7. P.Value：原始P值
8. adj.P.Val：经错误发现率校正的P值
9. CHR：染色体（chromosome）
10. pos：基于hg38/GRCh38的基因组位置
11. UCSC_RefGene_Name：基于Illumina EPIC芯片注释文件v1.0 B4的基因注释

此外，本研究还开展了荟萃分析，其结果的汇总统计数据输出包含以下字段：
1. RSID：探针ID（Probe ID）
2. STUDY：该探针所涉及的研究或亚组数量
3. BETA_meta：系数估计值
4. SEBETA_meta：标准误
5. PVALUE_meta：原始P值
6. PVALUE_Q：Cochrane Q检验P值
7. Model：模型类型，包括固定效应（FE, fixed effect）或二阶随机效应（RE2, random effect）
8. PVALUE_meta_adj：经错误发现率校正的P值