Effects of digested onion extracts on intestinal gene expression using rat intestine slices. Rattus norvegicus

Rat small intestine precision cut slices were exposed for 6 hours to in vitro digested yellow (YOd) and white onion extracts (WOd) that was followed by transcriptomics analysis. The digestion was performed to mimic the digestion that in vivo takes place in the stomach and small intestine. The transcriptomics response of the rat small intestine precision cut slices was compared to that of human Caco-2 cells and the pig in-situ small intestinal segment perfusion. The microarray data for the human Caco-2 cells (GSE83893) and the pig in-situ small intestinal segment perfusion (GSE83908) have been submitted separately from the current data on rat intestine. The goal was to obtain more insight into to which extent mode of actions depend on the experimental model. A main outcome was that each of the three models pointed to the same mode of action: induction of oxidative stress and particularly the Keap1-Nrf2 pathway. Overall design: The dataset contains results of 12 microarrays. 3 arrays for saline_digest that can be considered as the reference to which the response of the in vitro digested yellow (YOd) and white onion extracts (WOd) can be compared. 3 arrays were used for in vitro digested yellow (YOd) and 3 arrays for in vitro digested yellow (YOd). In addition, 3 arrays (called DMSO) were included for an exposure without digestion enzymes. That is informative for the effect of the enzymes in the digestion mixture.

将大鼠小肠精密切片（precision cut slices）置于经体外消化的黄色洋葱提取物（YOd）与白色洋葱提取物（WOd）中孵育6小时，随后开展转录组学分析。本次消化实验旨在模拟体内胃与小肠内的生理消化过程。

将上述大鼠小肠精密切片的转录组学响应，与人源Caco-2细胞以及猪原位小肠段灌注模型的转录组响应进行对比分析。

人源Caco-2细胞的微阵列（microarray）数据（GSE83893）与猪原位小肠段灌注模型数据（GSE83908）已与本次大鼠肠道数据集分别提交至公共数据库。

本研究旨在深入解析实验模型对作用模式分析的影响程度。

核心研究结果表明，三种实验模型均指向一致的作用模式：氧化应激诱导，尤其是Keap1-Nrf2通路的激活。

整体实验设计：本数据集包含12组微阵列检测结果。其中3组为生理盐水消化对照（saline_digest），作为体外消化的黄色洋葱提取物（YOd）与白色洋葱提取物（WOd）处理组的参照样本；3组对应体外消化的黄色洋葱提取物（YOd）处理，另有3组同样对应体外消化的黄色洋葱提取物（YOd）处理。此外，还设置了3组无消化酶暴露的二甲基亚砜（DMSO）对照样本，用于评估消化混合液中酶组分的影响。