Bilayer lipid membranes tethered by commercial surfactants on electrochemical sensors. Influence of the density of anchor-harpoon molecules.

Phospholipids assemble the backbone of biological membranes and their simplified biomimetic models, in particular sparsely tethered bilayer membranes (stBLM) were established to mimic and study these fundamental architectures. We have developped new model of stBLM that avoid cumbersome chemistry being based on the grafting of commercial surfactants (Brij, CiEj...) able to tether the membrane and of PEO to maintain its fluidity to any conductor or semi-conductor. This proposal aim to shade some light on the role played by the hybrid cushion by resolving the structure of the lipid bilayer tethered via different functionalisaations that will explore two different concentrations of the tether molecules (surfactant) and different hybrid coatings (Brij / PEOn).

磷脂构成生物膜及其简化仿生模型的骨架，其中稀疏锚定双层膜（sparsely tethered bilayer membranes，简称stBLM）被建立以模拟和研究这些基础结构。我们开发了一种新的stBLM模型，该模型避免了繁琐的化学步骤——其原理是将能够锚定膜的商用表面活性剂（Brij、CiEj...）和用于维持膜流动性的PEO接枝到任意导体或半导体上。本研究旨在通过解析经不同功能化修饰的脂质双层结构，阐明混合缓冲层的作用——这些功能化修饰将探索两种不同浓度的锚定分子（表面活性剂）以及不同的混合涂层（Brij/PEOn）。