Corticosteroid responsive genes. Rattus norvegicus

Three groups of Wistar rats with different manipulated levels of corticosterone were used to identify corticosteroid-responsive genes in hippocampus: 1. rats were adrenalectomised (ADX) 2. In addition to ADX these rats were implanted with a subcutaneous pellet containing 20 mg corticosterone and 80% cholesterol (ADX + low CORT) 3. In addition to ADX and implantation of a corticosterone pellet, these rats received a subcutaneous injection with corticosterone (1 mg/kg bodyweight) three hours prior to decapitation ((ADX + low CORT) + acute high CORT). All 3 groups of rats were decapitated three days after ADX and their hippocampus was isolated. Hippocampal RNA from 6 rats per group was pooled and used as input material for the SAGE libraries. Keywords: parallel sample

本研究选取三组皮质酮水平经差异化调控的Wistar大鼠，用于鉴定海马组织中糖皮质激素应答基因：1. 肾上腺切除（adrenalectomised，ADX）组大鼠；2. 于肾上腺切除基础上，皮下植入含20mg皮质酮与80%胆固醇颗粒（pellet）的ADX+低剂量皮质酮组（ADX + low CORT）；3. 在肾上腺切除并植入皮质酮颗粒的前提下，于断头处死前3小时皮下注射1mg/kg体重剂量皮质酮的（ADX + low CORT）+ 急性高剂量皮质酮组（(ADX + low CORT) + acute high CORT）。所有三组大鼠均在肾上腺切除术后3天实施断头处死，并分离其海马组织。每组取6只大鼠的海马体RNA进行混合，作为构建基因表达系列分析（Serial Analysis of Gene Expression, SAGE）文库的起始原料。关键词：平行样本