Representative mutants showing growth defects in low zinc.

Yeast pools were grown in zinc-replete (LZM+100 µM ZnCl2) and zinc-limiting (LZM+1 µM ZnCl2) media for either five or fifteen generations. The yeast ORFs/genes correspond to representative deletion strains that exhibited a significant change in growth in low zinc when compared to zinc-replete conditions (q<0.05). Numeric values are fitness scores (log2 ratios); a negative value indicates a growth defect of the mutant in low zinc. Empty cells in the table indicate that any differences observed were not significant at that generation point.
aAverage fitness scores for representative sensitive strains in four replicate experiments after 5 generations of growth.
bAverage fitness scores for representative sensitive strains in four replicate experiments after 15 generations of growth.

本数据集的酵母混合菌株分别在锌充足（LZM+100 µM ZnCl₂）和限锌（LZM+1 µM ZnCl₂）培养基中传代5代或15代。本次涉及的酵母开放阅读框（ORFs）/基因对应代表性缺失突变株，这些突变株在低锌条件下的生长表现与锌充足条件相比存在显著差异（q<0.05）。表格中的数值为适应度评分（log₂比值）；负值代表该突变株在低锌环境中存在生长缺陷。表格内的空单元格表示，在对应传代节点未观测到显著差异。
注a：传代5代后，通过4次重复实验测得的代表性敏感菌株的平均适应度评分。
注b：传代15代后，通过4次重复实验测得的代表性敏感菌株的平均适应度评分。