Light dependent gene expression in D. shibae wildtype compared to the gene expression in the transposonmutants D. shibae Dshi_1135::Tn, D. shibae ppaA::Tn and D. shibae ppsR::Tn
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE244289
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Light dependent gene expression in D. shibae wildtype compared to the gene expression in the transposonmutants D. shibae Dshi_1135::Tn, D. shibae ppaA::Tn and D. shibae ppsR::Tn. Dinoroseobacter shibae DFL12T (DSM 16493T) and the transposonmutants D. shibae Dshi_1135::Tn, D. shibae ppaA::Tn and D. shibae ppsR::Tn were grown in artificial saltwater minimal medium (SWM) in baffled flasks shaking at 180 rpm and 30 °C and incubation was performed under light, dark and bluelight conditions. D. shibae wild type and mutant strain were grown under aerobic conditions up to the mid exponential growth phase (OD578 nM 0.5). Dinoroseobacter shibae DFL12T (DSM 16493T) and the transposonmutants D. shibae Dshi_1135::Tn, D. shibae ppaA::Tn and D. shibae ppsR::Tn were grown in artificial saltwater minimal medium (SWM) in baffled flasks shaking at 180 rpm and 30 °C and incubation was performed under light, dark and bluelight conditions. D. shibae wild type and mutant strain were grown under aerobic conditions up to the mid exponential growth phase (OD578 nM 0.5).
本数据集旨在对比施氏玫瑰杆菌(*Dinoroseobacter shibae*)野生型与其转座子突变体(transposonmutant)的光依赖性基因表达谱,所涉突变株包括D. shibae Dshi_1135::Tn、D. shibae ppaA::Tn及D. shibae ppsR::Tn。
施氏玫瑰杆菌DFL12^T(DSM 16493^T)及其上述转座子突变株,均接种于人工海水最小培养基(SWM)中,于带挡板摇瓶内以180转/分钟、30℃条件下振荡培养,培养环境分别设置为光照、黑暗及蓝光。野生型菌株与突变株均在有氧条件下培养至指数生长中期(OD578 nM 0.5)。
施氏玫瑰杆菌DFL12^T(DSM 16493^T)及其转座子突变株D. shibae Dshi_1135::Tn、D. shibae ppaA::Tn、D. shibae ppsR::Tn,均接种于人工海水最小培养基(SWM)中,于带挡板摇瓶内以180转/分钟、30℃条件下振荡培养,培养环境分别设置为光照、黑暗及蓝光。野生型菌株与突变株均在有氧条件下培养至指数生长中期(OD578 nM 0.5)。
创建时间:
2024-03-07



