Expression data from CSC and Non-CSC of three HCC cell lines

Hepatocellular carcinoma (HCC) represents the major subtype of liver cancer, characterized with a high rate of recurrence and heterogeneity. Liver cancer stem cells (CSCs) may account for a hierarchical organization of heterogeneous cancer cells. However, how liver CSCs sustain their self-renewal remains largely unknown. We used microarrays to discover the long non-coding RNAs (lncRNAs) expression underlying cell stem cell (CSC) and non cell stem cell (non-CSC) and identified distinct lncRNAs during this process. We sorted CD13+CD133+ and CD13-CD133- cells from Hep3B, Huh7, and PLC/PRF/5 HCC cell lines as liver CSCs and non-CSCs, then hybridized on Affymetrix microarrays. We sought to identify distinct lncRNAs in liver CSCs.

肝细胞癌（Hepatocellular carcinoma, HCC）是肝癌的主要亚型，以高复发率与显著异质性为典型特征。肝癌干细胞（Cancer Stem Cells, CSCs）被认为是驱动异质性肿瘤细胞形成层级组织结构的关键群体，但目前关于肝癌干细胞如何维持其自我更新能力的机制仍不甚明晰。本研究借助基因芯片技术，探究了肝癌干细胞（CSCs）与非干细胞样肝癌细胞（non-CSCs）的长链非编码RNA（long non-coding RNAs, lncRNAs）表达差异，并在此过程中筛选得到一批差异表达的lncRNAs。具体实验流程为：从Hep3B、Huh7及PLC/PRF/5三株肝细胞癌细胞系中分选出CD13+CD133+与CD13-CD133-细胞，分别作为肝癌干细胞与非干细胞样肝癌细胞，随后将两类细胞的样本进行Affymetrix基因芯片杂交，旨在明确肝癌干细胞中特异性表达的长链非编码RNA。