Image analysis of liver cell nuclei from sodium valproate-treated developing rats

The data set identifies nuclear phenotype characteristics of rat liver cells looking for changes associated with sodium valproate (VPA) treatment during animal development. Liver imprints were obtained from male Wistar rats that after a 21-day age received 100 mg/kg VPA daily for 9, 14 and 39 days. The preparations were fixed in an absolute ethanol-acetic acid mixture (3:1, v/v) for 5 min and subjected to the Feulgen reaction for studying nuclear features evaluated with image analysis using KS400-version 3 software. The nuclear features obtained were as follows: total area (µm2), absorbing area (µm2), perimeter (µm), Feret ratio, optical density (= absorbance), integrated optical density, variability in the degree of chromatin packaging (SDtd), nuclear entropy, and nuclear energy. Animal care and use protocols were approved by the Ethics Committee for Animal Use at the University of Campinas (registration no. 6269-1/2023) and followed the guidelines established by the Brazilian National Council for the Control of Animal Experimentation (CONCEA).

本数据集旨在识别大鼠肝细胞的核表型特征，探究动物发育过程中与丙戊酸钠（sodium valproate, VPA）处理相关的变化。样本来源于雄性Wistar大鼠，这些大鼠在21日龄后每日接受100mg/kg的VPA处理，处理时长分别为9天、14天和39天。样本采用无水乙醇-乙酸混合液（体积比3:1）固定5分钟，随后进行福尔根反应（Feulgen reaction）以研究核特征；核特征的评估通过KS400-3.0版软件进行图像分析完成。获得的核特征包括：总面积（µm²）、吸收面积（µm²）、周长（µm）、费雷特比（Feret ratio）、光密度（即吸光度）、积分光密度、染色质包装程度的变异性（SDtd）、核熵及核能。动物护理与使用方案已获得坎皮纳斯大学动物使用伦理委员会批准（注册号：6269-1/2023），并遵循巴西国家动物实验控制委员会（CONCEA）制定的指南。