Genome-wide analysis of LPS-responsive gene expression by microglia cells in Ntg and LRRK2KO mouse brain. Mus musculus

Analysis of LRRK2-regulation of microglia responce to the LPS at gene expression level. The hypothesis tested in the present study was whether LRRK2 influence the microglial phagocytosis- and neuroinflammation- related gene expression at mRNA level. Overall design: Total RNA obtained from microglia cells isolated from Ntg or LRRK2KO mouse brain subjected to 6 or 24 hours of LPS treatment in vitro

本研究针对基因表达水平下LRRK2对脂多糖（Lipopolysaccharide, LPS）诱导的小胶质细胞（microglia）应答的调控作用展开分析。本研究所验证的科学假说为：LRRK2是否可在信使RNA（messenger RNA, mRNA）层面，调控小胶质细胞中与吞噬作用及神经炎症相关的基因表达。整体实验设计：从非转基因（non-transgenic, Ntg）或LRRK2基因敲除（LRRK2 knockout, LRRK2KO）小鼠脑组织中分离的小胶质细胞，经体外LPS分别处理6小时与24小时后，提取其总RNA。