The polymorphism of the broomrape generated by ISSR primers

As a holoparasitic weed, broomrape has seriously threatened the production of economically important crops, such as melon, watermelon, processed tomato, and sunflower, in Xinjiang Province in recent years. However, the distribution and genetic diversity of broomrape populations in Xinjiang are not clear at present, which hinders their prevention and control process. In the present study, 93 samples from different geographic regions of Xinjiang were collected to identify the species based on ITS and plastid rps2 regions, and the samples were used to analyze the genetic diversity based on ISSR markers. The results showed that broomrape is not monophyletic in Xinjiang and consists of two major clades (Orobanche cf. aegyptiaca and O. cernua) and three subclades (O. cf. aegyptiaca var. tch, O. cf. aegyptiaca var. klz, O. cernua.var. alt) by phylogenetic analysis based on ITS and rps2. Furthermore, the results of the genetic diversity analysis indicated that the 11 selected primers produced 1..., DNA extraction and PCR amplification
Total DNA was extracted from all samples following the cetyl-trimethylammonium bromide (CTAB) method described by Xin and Chen (Xin and Chen, 2012). The purity of the DNA was determined by 1.0% agarose gel electrophoresis and absorption at 260 nm, and the ratios A260/А280 and A260/А230 were used to determine the presence of contaminants such as proteins, polyphenolic compounds, sugars, and lipids. The samples were diluted to 50 ng/μl for PCR amplification.
The ITS sequences of the partial samples were amplified with primer pair QR-F (5’- CCTGCAAAAGCAGACCGT-3′) and QR-R (5’-CGCAATCGAAGGCACGAG-3′), which were designed based on the ITS alignment of O. aegyptiaca (AY209327.1). Other ITS sequences were amplified with the primer pair ITS1 (5’-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-TCCTC CGCTTATTGATATGC-3′) (Kwon et al., 2012). PCR amplification was carried out on a DNA engine dyad® Peltierp thermal cycler (Bio-Rad) using a 50 μL reaction mixture containing 1..., , # The polymorphism of the broomrape generated by ISSR primers

[https://doi.org/10.5061/dryad.xd2547dpk](https://doi.org/10.5061/dryad.xd2547dpk)

## Description of the data and file structure

The 11 ISSR primers were obtained by screening and generated a total of 154 bands among 93 samples, of which 150 bands were polymorphic.

## Sharing/Access information

No

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## Code/Software

Lasergene DNASTAR (version 6.0) , DNAMAN (version 6.0), MEGA6.0, POPGENE version 1.32, and  Numerical Taxonomy Multivariate Analysis System version 2.10

近年来，列当（broomrape）作为一种全寄生性杂草，已对我国新疆地区的甜瓜、西瓜、加工番茄、向日葵等经济作物的生产造成严重威胁。但目前新疆地区列当种群的分布与遗传多样性尚不明晰，这极大阻碍了其防控工作的推进。本研究采集了来自新疆不同地理区域的93份列当样本，基于内部转录间隔区（Internal Transcribed Spacer, ITS）序列与质体rps2基因区域进行物种鉴定，并利用简单序列重复区间扩增多态性（Inter-Simple Sequence Repeat, ISSR）标记开展遗传多样性分析。

基于ITS与rps2序列的系统发育分析结果显示，新疆地区的列当并非单系类群，可分为两大演化支（埃及列当近似种*Orobanche cf. aegyptiaca*与弯管列当*O. cernua*）以及三个亚演化支（埃及列当近似种tch变种*O. cf. aegyptiaca var. tch*、埃及列当近似种klz变种*O. cf. aegyptiaca var. klz*，以及弯管列当alt变种*O. cernua var. alt*）。此外，遗传多样性分析结果显示，所筛选的11对引物共扩增出1……

DNA提取与PCR扩增
所有样本的总DNA均参照Xin与Chen（2012）所述的十六烷基三甲基溴化铵（CTAB）法进行提取。DNA纯度通过1.0%琼脂糖凝胶电泳以及260 nm吸光度值进行检测，并利用A260/A280与A260/A230比值判断是否存在蛋白质、多酚类化合物、糖类及脂质等污染物。将DNA样品稀释至50 ng/μl用于PCR扩增。

部分样本的ITS序列采用基于埃及列当（*O. aegyptiaca*，AY209327.1）ITS序列比对设计的引物对QR-F（5’- CCTGCAAAAGCAGACCGT-3′）与QR-R（5’-CGCAATCGAAGGCACGAG-3′）进行扩增；其余ITS序列则采用引物对ITS1（5’-TCCGTAGGTGAACCTGCGG-3′）与ITS4（5’-TCCTCCGCTTATTGATATGC-3′）进行扩增（Kwon等，2012）。PCR扩增在DNA Engine Dyad® Peltier型热循环仪（Bio-Rad）上进行，反应体系为50 μL，包含1……

# ISSR引物扩增的列当多态性分析
[https://doi.org/10.5061/dryad.xd2547dpk](https://doi.org/10.5061/dryad.xd2547dpk)

## 数据与文件结构说明
本研究筛选得到11条ISSR引物，在93份样本中共扩增出154条条带，其中150条为多态性条带。

## 共享与获取说明
无

## 代码与软件
Lasergene DNASTAR（版本6.0）、DNAMAN（版本6.0）、MEGA6.0、POPGENE版本1.32，以及数值分类多元分析系统（Numerical Taxonomy Multivariate Analysis System）版本2.10