Evaluation of strategic control of caprine arthritis- encephalitis in dairy herd goats

ABSTRACT The aim of this study was to evaluate the periodic use of more sensitive diagnostic tests associated to management practices for the effective control of caprine arthritis-encephalitis (CAE). We carried out eight blood samples in does and bucks. From the first to the seventh analysis, the samples were quarterly, using Agarose Gel Immunodiffusion (AGID), Enzyme linked immunosorbent assay (i-ELISA) and Western Blot (WB) tests. The eighth collection was made six months after the seventh, using the WB and Polymerase Chain Reaction (PCR). The prevalence of CAE was 6.8%, 14.9% and 39.2% in the AGID, i-ELISA and WB respectively. The last analysis detected 0.9% of animals positive by WB and 10.8% by PCR. Although they do not eradicate CAE, steps taken together with the periodic use of serological tests and the combination with PCR were important to significantly reduce positive animals in the herd.

摘要
本研究旨在评估将高灵敏度诊断检测方法与饲养管理措施联用并定期实施的方案，以实现山羊关节炎-脑炎（caprine arthritis-encephalitis, CAE）的有效防控。我们共采集了8份来自母山羊与公山羊的血液样本。在第1至第7次检测中，每季度开展一次采样，采用琼脂凝胶免疫扩散试验（Agarose Gel Immunodiffusion, AGID）、酶联免疫吸附试验（Enzyme linked immunosorbent assay, i-ELISA）以及蛋白质印迹试验（Western Blot, WB）开展检测。第8次样本采集于第7次采样6个月后，采用蛋白质印迹试验（WB）与聚合酶链式反应（Polymerase Chain Reaction, PCR）进行检测。AGID、i-ELISA与WB检测对应的CAE阳性率分别为6.8%、14.9%与39.2%。末次检测中，经WB检测的阳性动物占比为0.9%，经PCR检测的阳性动物占比为10.8%。尽管此类方案无法根除山羊关节炎-脑炎，但定期联合应用血清学检测手段并结合聚合酶链式反应（PCR），可显著降低畜群中的阳性动物占比，具有重要的实践意义。