THE EFFECT OF MUSCLE DAMAGE AND THE IL-6-174C/G POLYMORPHISM ON THE SERUM IL-6 LEVELS OF OLDER MEN

ABSTRACT Introduction Fast population aging is a global reality. Today’s major challenge is to promote the healthy aging of more and more people by acting on factors that can be modified, such as physical exercise. Regular exercise could contribute to the prevention of chronic diseases associated with aging. Research has been conducted on the physical training response of elderly individuals, but there is not yet any consensus on the influence of strength training or IL-6 polymorphism on levels of inflammatory markers such as IL-6 and muscle damage marker CK, particularly in healthy elderly male individuals. Objectives The aim of this study was to evaluate the relationship of IL-6 promoter -174 C/G gene polymorphism on systemic IL-6 responses and muscle damage after eccentric strength training in elderly men. Methods This is a prospective, high-quality study. Gene frequency of polymorphism of promoter gene IL6 G-174C was identified using the Hardy-Weinberg test in 28 older male volunteers. The relationships of each genotype with IL-6 and CK serum levels were analyzed. CK and IL-6 levels were determined at pre-training and 0h, 3h, 24h, and 48h post-training periods. Results Differences in baseline and post-training IL-6 levels of genotypic groups were observed for all time periods analyzed (p = 0.029). Eccentric exercise efficiently reduced post-intervention muscle damage, thus showing a statistical difference between the pre- and post-intervention time points ( p = <0.0005). Conclusion Eccentric training influenced CK and IL-6 modulation independently of the polymorphism of the IL-6 promoter gene -174 C/G. Level of evidence II, Prospective comparative type.

摘要
引言
人口快速老龄化是全球性的客观现实。当前的核心挑战在于，通过干预可调控的风险因素（如体育锻炼），推动越来越多的老年人实现健康老龄化。规律运动有助于预防与衰老相关的慢性疾病。目前学界已针对老年人的运动训练应答开展相关研究，但针对力量训练或白细胞介素-6（IL-6）基因多态性对IL-6等炎症标志物及肌酸激酶（CK）等肌肉损伤标志物水平的影响尚未达成共识，针对健康老年男性群体的相关研究尤为匮乏。
研究目的
本研究旨在探讨IL-6启动子-174 C/G基因多态性与老年男性离心力量训练后全身IL-6应答水平及肌肉损伤程度的关联。
研究方法
本研究为前瞻性高质量研究。采用哈迪-温伯格（Hardy-Weinberg）平衡检验，对28名老年男性志愿者的IL6 G-174C启动子基因多态性的基因频率进行鉴定。分析不同基因型与血清IL-6及CK水平的关联。分别于训练前、训练后0小时、3小时、24小时及48小时检测IL-6与CK血清水平。
研究结果
在所分析的全部时间节点中，各基因型组的基线及训练后IL-6水平均存在统计学差异（p=0.029）。离心力量训练可有效降低干预后的肌肉损伤程度，干预前后的时间节点间存在显著统计学差异（p<0.0005）。
研究结论
离心力量训练可对CK与IL-6的表达调控产生影响，且该效应不受IL-6启动子-174 C/G基因多态性的影响。
证据等级：II级，前瞻性对照研究类型。