ASSISTED REPRODUCTIVE TECHNOLOGIES ALTER DNA METHYLATION PROFILES IN BLOODSPOTS OF NEWBORN INFANTS

The Illumina Infinium HumanMethylation 450k Beadchip was employed to study the impact of in-vitro fertilization on DNA methylation in peripheral blood of infants. Using a total of 137 Michigan newborns born through IVF using fresh embryo transfer, IVF using cryopreserved embryo transfer, Intrauterine Insemination, or unassisted conception, DNA from archived Guthrie cards was assayed for methylation profiles. A study in differential methylation, DNA from 137 archived bloodspots were bisulphite converted and assayed using the Infinium HumanMethylation 450k Beadchip

本研究采用Illumina Infinium人类甲基化450K芯片（Illumina Infinium HumanMethylation 450k Beadchip），探究体外受精（in-vitro fertilization, IVF）对婴儿外周血DNA甲基化的影响。本研究共纳入137名密歇根新生儿，其受孕方式涵盖新鲜胚胎移植体外受精、冻存胚胎移植体外受精、宫腔内人工授精（Intrauterine Insemination, IUI）或自然受孕；研究人员对存档的格思里采血卡（Guthrie card）中的DNA开展甲基化谱分析。在差异甲基化分析中，研究人员对137份存档的血斑DNA进行亚硫酸氢盐转化处理，并采用Illumina Infinium人类甲基化450K芯片完成检测。