In vitro generation of long-lived human Plasma Cells

Gene expression profiling of B-cells from a model differentiation series: from Naïve B-cells, through a proliferative plasmablast stage to long-lived antibody secreting plasma cells. B-cells were isolated from the peripheral blood of three adult donors and differentiated in vitro (see individual samples for culture conditions)

本数据集针对一套经典B细胞分化模型的基因表达谱展开分析：样本涵盖从初始B细胞（Naïve B-cells）、增殖性成浆细胞（plasmablast）阶段直至长寿抗体分泌型浆细胞（antibody secreting plasma cells）的完整分化序列。研究人员从3名成年供者的外周血中分离B细胞，并在体外完成分化培养，具体培养条件详见各单个样本的说明。